Ergosterol supplementation improves furfural tolerance of Saccharomyces cerevisiae to produce ethanol and its underlying mechanism
نویسندگان
چکیده
Furfural produced during lignocellulose pretreatment to reduce the recalcitrance inhibits growth of Saccharomyces cerevisiae and reduces ethanol yield. To adverse effect furfural on S. cerevisiae, exogenous ergosterol was supplemented impact under stress studied. The lag phage shortened by 50%, maximum yield increased 158% with 50 mg/L supplementation 4 g/L stress. Flow cytometry analysis results showed that permeable cells intracellular reactive oxygen species were decreased 45 53%, respectively addition fatty acid composition changed; glycerol content after supplementation. saturation increased. Addition promoted cell decreasing oxidative Under stress, BY4741 (erg3?) (erg5?) longer than BY4741, concentration decreased.
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ژورنال
عنوان ژورنال: Bioresources
سال: 2022
ISSN: ['1930-2126']
DOI: https://doi.org/10.15376/biores.18.1.228-246